Corrected and Approved By B.T.E. On Dated 10.06.2015
1.1 ANALYTICAL BIOCHEMISTRY
1. General principles of analytical biochemistry.
2. The concept of pH, Properties of water, dissociation andionization of acids and bases, pKa, buffers and buffering mechanisms, dissociations of amino acids and determination of pKa.
3. Structure of proteins and metabolism, carbohydrates and lipids.
4. Chromatography: principles and application of adsorption,partition, ion-exchange, gel filtration, affinity and high performance liquid chromatography.
5. Electrophoresis : moving boundry and zonal electrophoresis,paper and gel electrophoresis, isoelectric focusing technique.
6. Gel electrophoresis and centrifugation techniques and application
7. Electroanalytical methods.
8. Principles and applications of ultraviolet and visible light spectrophotometry, NMR spectroscopy, spectrofluorometry and atomic spectroscopy, mass spectroscopy.
9. Tracer technices, ELISA, RIA, IRMA, Scintillation counters.
LIST OF PRACTICALS
1. To prepare various buffer solutions required for tissue culture and bio-chemical work.
2. Separation of leaf pigment adsorption chrnatigraogy.
3. Seperation of amino acids by TLC.
4. Qualitative and quantitative analysis of carbohydrates.
5. Determination of acid value of fat.
6. Determination of iodine number of fat.
7. Qualitative and quantitative analysis of proteins.
8. Estimation of Enzyme activity.
9. Protein purification by gel filtration chromate graphy.
10. Molecular weight determination of protein through SDS PAGE.
11. Centrifugation and separation of cell components.
1.2 MOLECULAR BIOLOGY AND GENETIC ENGINEERING
1. Introduction, Development, Present Status and Prospects ofMolecular Biology and Genetic Engineering.
2. Replication of DNA,Types, Structure and properties of DNA and RNA and the genetic code.
3. Genome organization in viruses, Prokaryotes and eukaryotes.
4. Gene Expression; Central Dogma,Transcription, Translation,Regulation of Gene expression.
5. A brief account of repetitive DNA sequences, split genes,transposons.
6. Introduction to gene mapping, Genomics and Metabolomics,ionomics , phenomics and proteomics.
7. Fundamentals of recombinent DNA technology: DNA extraction,labelling of nucleic acids, Restriction endonucleases, cloning vectors, Isolation and cloning of genes,gene libraries.
8. Fundamentals of molecular techniques and their uses PCR,Agarose gel electrophoresis, documentation and blotting techniques.
1. Isolation of plasmid DNA from E. Coli. (Transformed) cells.
2. Quantitation of nucleic acids by gel electrophoresis and spectrophotometry.
3. Isolation of DNA from plant and microbial cells.
4. Spectrophotometric Determination of melting temperature of DNA
5. Demonstration of digestion of DNA by restriction endonuclease and agarose gel electrophoresis.
6. Isolation of RNA.
7. Mutation in yeast by physical multagenic agents.
1.3 INDUSTRIAL MICROBIOLOGY
1. Microbial growth and nutrition, Fermentation Media.
2. Microbial metabolism, Glycolysis, TCA and Secondary metabolism path way.
3. Classes and properties of micro-organisms relevant to water, food, alcohol, dairy bio fertilizer and bio-operative industries etc.Industrial microorfanisms,important for water, food alcohol, dairy, biofertilizer biopesticide industries, etc..
4. Fermentation systems, Aerobic and anaerobic, Solid and submerged, Surface culture.
5. Downstream processing, Bioreactors – types and uses. step and methods.
6. Microbial enzymes, Pralease, Amylates, Lipates and their appliaction.
7. Fuels and industrial products Ethanal, Acetone, Butanol.
8. Health care products, Therapeutic and Antibiatics.
9. Food and beverages fermentaions Systems.
10. Food additives, Vitamins and Aminoacids.
11. Down Stream processing : Steps and Methods.
12. Waste water treatment : Characterization techniques and Methods.
LIST OF PRACTICALS
1. Sterilization of glasswares and preparation of culture media for microbial growth.
2. Simple and differential stanining of different microorganisms.
3. Microbial growth measurement by different dry weight (Counting, O D., measurement).
4. Effect of pH temperature and substrate conc. on growth of microorganisms.
5. Fermentative production of alcohol.
6. Determination of total dissolved solids of water.
7. Determination of biological oxygen demand (BOD) of water.
8. Determination of chemical oxygen demand (COD) of water.
9. Colony characterzation of micro organisms.
10. Microscopit determination of shape and size of yeast cell.
11. Screening and isolation of Azato dictes.
12. Maintenance of culture on agar medium.
13. Production of invertase enzyme.
plant tissue culture technology
1. Introduction: Definition, history, present status, future prospects of tissue culture, Cell totipotency.
2. Composition of different culture Media, Role of chemicals and growth regulators in tissue culture.
3. Sterilization of media, tissues and other accessories, Inoculation of cultures.
4. Callus Induction and plant regeneration. Organogenesis and Embryogensis.
5. Suspension culture, Protoplast isolation, culture and fusion.
6. Clonal propagation : Shoot-tip and axillary bud culture.
7. Techniques of cell and tissue culture: Preparation of explant materials, initiation of cultures, micropropagation through organogensis and embryogensis, artificial seeds.
8. Anther Culture: Development of haploids, diploidization and its applications.
9. Embryo culture.
10. Somaclonal variations and in vitro selections towards crop improvement.
11. Industrial appliction of plant tissue culture for production of Secondary metabolites.
12. Plant biotechnology and crop improvement for grain quality, fiber quality, disease resistance, insect resistance, herbicide resistance, stress resistance (basic outlines).
1. Orientation : Introduction to tissue culture laboratory; washing chamber, media preparation laboratory, transfer laboratory, sterilization laboratory inoculation laboratory, culture room.
2. Tissue culture requirements; glass ware, water distillation Unit, chemicals, instruments; Autoclave, pH meter, sterile air flow chamber (Laminar flow).
3. Preparation of Media.
4. Sterlization: Media, Explant,glassware.
5. Inoculation, Callus Induction and clonal propagation.
6. Subculturing and Regeneration of roots, shoots and plants.
7. Preparation of regenerated plants for hardening.
8. Protoplast Isolation and culture(demonstration).
9. Induction of embryogenic callus and encapsulation of artificial seeds.
10. Anther culture: Regeneration of plants from anthers/pollens.
11. Agrobacterium – mediated transformations (demonstration).
1. Environmental parameters for optimum plant growth.
2. Photoperiodism, vernalization.
3. Green House and polyhouse materials, designs & fabrication.
4. Cooling, shading, misting, drip irrigation,fertilization and fumigation.
5. Recording and Computerisation of environmental parameters and cultural operations.
7. Pots, potting mixtures and soil sterilization.
8. Cultivation of Horticultural plants.
9. Hardening of tissue culture derived horticultural plants.
10. Post harvest technology.
11. Packaging and transportation.
12. Intellectual Property Rights.
1. Raising of off-season nursery in poly houses.
2. Preparation of tissue culture derived plants for hardening.
3. Preparation of soil mix and sterilization.
4. Hardening of tissue culture derived plants.
5. Soil culture and hydroponics, sand culture of roses and
selected vegetables in green house.
6. Harvesting, preparation, cooling of cut flowers and vegetables.
7. Packing and transportation (Demonstration only).
Environmental education and disaster management
TOPIC WISE DISTRIBUTION OF PERIODS:
SL. NO. TOPIC L T P
1. Introduction 6
2. Pollution 3
2.1 Water Pollution 8
2.2 Air Pollution 8
2.3 Noise Pollution 3
2.4 Radio Active Pollution 4
2.5 Solid Waste Management 5
3. Legislations 3
4. Environmental Impact Assessment 4
5. Disaster Management 6
TOTAL 50 – -
1. INTRODUCTION :
– Basics of ecology, Ecosystem, Biodiversity Human activities and its effect on ecology and eco system, different development i.e. irrigration, urbanization, road development and other engineering activities and their effects on ecology and eco system, Mining and deforestation and their effects. – Lowering of water level , Urbanization. – Biodegradation and Biodegradibility, composting, bio remediation, Microbes .Use of biopesticidies and biofungicides. – Global warning concerns, Ozone layer depletion, Green house effect, Acid rain,etc.
2. POLLUTION :
Sources of pollution, natural and man made, their effects on living environments and related legislation.
2.1 WATER POLLUTION :
– Factors contributing water pollution and their effect.
– Domestic waste water and industrial waste water. Heavy
metals, microbes and leaching metal.
– Physical, Chemical and Biological Characteristics of waste
– Indian Standards for qulity of drinking water.
– Indian Standards for quality of treated waste water.
– Treatment methods of effluent (domestic waste water and
industrial/ mining waste water), its reuse/safe disposal.
2.2 AIR POLLUTION :
Definition of Air pollution, types of air pollutants i.e.
SPM, NOX, SOX, GO, CO2, NH3, F, CL, causes and its effects
on the environment.
– Monitoring and control of air pollutants, Control measures
techniques. Introductory Idea of control equipment in
A. Settling chambers
C. Scrubbers (Dry and Wet)
D. Multi Clones
E. Electro Static Precipitations
F. Bog Fillers.
– Ambient air qulaity measurement and their standards.
– Process and domestic emission control
– Vehicular Pollution and Its control with special emphasis of
Euro-I, Euro-II, Euro-III and Euro IV.
2.3 NOISE POLLUTION :
Sources of noise pollution, its effect and control.
2.4 RADIOACTIVE POLLUTION :
Sources and its effect on human, animal, plant and material,
means to control and preventive measures.
2.5 SOLID WASTE MANAGEMENT :
Municipal solid waste, Biomedical waste, Industrial and
Hazardous waste, Plastic waste and its management.
3. LEGISLATION :
Preliminary knowledge of the following Acts and rules made
– The Water (Prevention and Control of Pollution) Act – 1974.
– The Air (Prevention and Control of Pollution) Act – 1981.
– The Environmental Protection (Prevention and Control of
Pollution) Act -1986. Rules notified under EP Act – 1986
# The Manufacture, Storage and Import of Hazardous
Chemical (Amendment) Rules, 2000
# The Hazardous Wastes (Management and Handling )
Amendment Rules, 2003.
# Bio-Medical Waste (Management and Handling) (Amendment)
# The Noise Pollution (Regulation and Control)
(Amendment) Rules, 2002.
# Municipal Solid Wastes (Management and Handling) Rules,
# The Recycled Plastics Manufacture and Usage (Amendment)
4. ENVIRONMENTAL IMPACT ASSESSMENT (EIA) :
– Basic concepts, objective and methodology of EIA.
– Objectives and requirement of Environmental Management
System (ISO-14000) (An Introduction).
5. DISASTER MANAGEMENT :
Definition of disaster – Natural and Manmade, Type of
disaster management, How disaster forms, Destructive power,
Causes and Hazards, Case study of Tsunami Disaster, National
policy- Its objective and main features, National
Environment Policy, Need for central intervention, State
Disaster Authority- Duties and powers, Case studies of
various Disaster in the country, Meaning and benifit of
vulnerability reduction, Factor promoting vulnerability
reduction and mitigation, Emergency support function plan.
Main feature and function of National Disaster
Management Frame Work, Disaster mitigation and prevention,
Legal Policy Frame Work, Early warning system, Human
Resource Development and Function, Information dissemination and communication